【公開日：2025.06.10】【最終更新日：2025.04.22】
課題データ / Project Data
課題番号 / Project Issue Number
24NM5509
利用課題名 / Title
Monitoring of heavy metals in living cells using nanomaterials
利用した実施機関 / Support Institute
物質・材料研究機構 / NIMS
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）物質・材料合成プロセス/Molecule & Material Synthesis（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）マテリアルの高度循環のための技術/Advanced materials recycling technologies（副 / Sub）-
キーワード / Keywords
nanomaterials, toxicity of heavy metals, MTT assay,赤外・可視・紫外分光/ Infrared/visible/ultraviolet spectroscopy
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
Islam El-Sewify
所属名 / Affiliation
物質・材料研究機構
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）,機器利用/Equipment Utilization
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
This study investigates nanomaterials for detecting and monitoring heavy metals in living cells. Nanomaterials possess enhanced surface area, optical properties, and interactions with biological molecules, enabling real-time monitoring and providing insights into cellular absorption, distribution, and toxicity. Heavy metals such as lead, mercury, and cadmium can be detected using colorimetric assessments and fluorescence spectroscopy. These techniques offer real-time monitoring and provide insights into the absorption, distribution, and toxicity of heavy metals in cells.
実験 / Experimental
The Hela cells were seeded and incubated in Dulbecco's Modified Eagle's Medium under CO2 at 37°C. The culture medium was discarded and replaced to increase the HeLa cell population. The MTT protocol was conducted to investigate the cytotoxicity of the designed nanomaterial. The cell viability was examined against HeLa cell lines using MTT assays to evaluate the cytotoxicity. The HeLa cell concentration was adjusted to be 50 × 104 cells per well and incubated in a medium containing MFT (0-50 μg/ml) at concentrations ranging from 24 h. Furthermore, MTT (100 μL) was injected into each well and incubated for four h. During the incubation, formazan crystals were observed via the interaction of the MTT reagent with active cells. After that, the microplate was washed away to remove the extra MTT and medium. To dissolve the observed crystals, DMSO (100 μL) was stimulated in each well. The absorbance was recorded using a microplate reader.
結果と考察 / Results and Discussion
To evaluate the suitability of engineered nanomaterials in biological systems, we utilized a cytotoxicity and cell viability approach. Our findings confirmed that there was no cell membrane shrinkage or damage at the micro-scale of the nanomaterials. As a result, the modified nanomaterials with mesoporous channels show promise for visualizing, monitoring, and tracing metal ions poisoning in an in vitro model, thereby contributing to clinical diagnosis in biological cells.
図・表・数式 / Figures, Tables and Equations
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件